重组病毒载体,Recombinant virus vector
1)Recombinant virus vector重组病毒载体
1.At present,recombinant virus vector is one of the most hotspots in virus gene engineering,There are two vector type, the replaceable recombinant virus vector and the recombinant viral plasmid vector.重组病毒载体又是当今病毒基因工程研究的热点之一,目前以动物病毒为基础设计的基因克隆及表达载体主要有取代型的重组病毒载体和重组的病毒-质粒载体。
2.pgR107,X-group virus vector of potato was used and then RNA interference recombinant virus vector pVX-AnFtsZi was constructed.利用简并引物RT-PCR,克隆复叶槭FtsZ基因的cDNA序列,利用马铃薯X组病毒载体pgR107,构建RNA干扰重组病毒载体pVX-AnFtsZi,并转化农杆菌GV3101(含辅助质粒pJIC Sa-rep)侵染烟草。
2)Recombinant adenovirus vector重组腺病毒载体
1.Construction and identification of recombinant adenovirus vector containing Tet-on adjustable NT3 and BDNF genes;人NT3、BDNF基因Tet-on可调控重组腺病毒载体的构建和鉴定
2.Construction of recombinant adenovirus vector encoding NF-κB RNA aptamer;核转录因子κB RNA适体重组腺病毒载体的构建
3.Construction of recombinant adenovirus vector pAdBM5-GFP-hIL-2 and the detection of viral titer重组腺病毒载体pAdBM5-GFP-hIL-2的构建及病毒滴度测定
英文短句/例句
1.Construction and Identification of a Recombinant Adenovirus Vector Containing PPARΓ_2 Gene;PPARγ_2基因重组腺病毒载体的构建与鉴定
2.Construction and Identification of Recombinant Adenovirus Vector Carring Cytosine Deaminase Gene;重组腺病毒载体pAd-CD的构建和鉴定
3.Construction and Identification of Recombinant Adenovirus Vector Containing rhGM-CSF Gene;rhGM-CSF重组腺病毒载体的构建及鉴定
4.Construction of Recombinant Adenovirus Vector of CGRP;小鼠CGRP基因重组腺病毒载体的构建
5.Construction and Expression in Vitro of Recombinant Adenoviral Vector Expressing CRT/MAGE-A3CRT/MAGE-A3重组腺病毒载体的构建及表达
6.Construction of Recombination Adenovirus Expressing NF-κBp65-Specific siRNANF-κBp65特异性siRNA重组腺病毒载体的构建
7.Construction and identification of recombinant adenovirus vector Ad5-hBDNF-EGFP重组腺病毒载体Ad5-hBDNF-EGFP的构建与鉴定
8.Construction of Recombinant Adenovirus Vector Containing the Capsid and 3C or 3ABC Protease Coding Regions of Foot-and-Mouth Disease Virus;A型口蹄疫病毒多基因重组腺病毒载体的构建
9.Construction of recombinant adenovirus vectors carrying murine GATA3 gene and establishment of the adenovirus小鼠GATA3基因重组腺病毒载体的构建和病毒制备
10.Construction and Assemblage of Recombinant Adenoviral Vector Carrying Muscovy Duck Reovirus σC Encoding Gene;番鸭呼肠孤病毒σC编码基因重组腺病毒载体的构建与组装
11.Construction of recombinant adenovirus vector expressing the human IL-10人白细胞介素10重组腺病毒载体的构建
12.Construction and Identification of Recombinant Adenoviral Plasmid Expressing the N-terminal Domain of TIMP-3;TIMP-3 N端结构域重组腺病毒载体的构建与鉴定
13.Construction of Recombinant for Adenovirus Expression Vector Encoding IKK2dn Gene and Identification of Their Expression;携带IKK2dn基因重组腺病毒载体的构建及表达验证
14.Cloning of Goat △FosB Gene and Construction of Recomninant Adenoviral Vector山羊△FosB基因的克隆及重组腺病毒载体的构建
15.Construction and identification of replication recombinent adenovirus vector of mice LINGO-1 shRNA含鼠LINGO-1 shRNA重组腺病毒载体的构建和鉴定
16.Infecting Human Dendritic Cells with Ad5-EGFP Recombinant Adenovirus Vectors重组腺病毒载体Ad5-EGFP感染人树突状细胞的研究
17.Construction of recombinant adenovirus vectors expressing two different subtypes of ndrg2 genendrg2基因2种亚型重组腺病毒载体的构建
18.Construction and Identification of adenovirus vector encoding mouse PGC1α gene编码小鼠PGC1α基因的重组腺病毒载体构建及鉴定
相关短句/例句
Recombinant adenovirus vector重组腺病毒载体
1.Construction and identification of recombinant adenovirus vector containing Tet-on adjustable NT3 and BDNF genes;人NT3、BDNF基因Tet-on可调控重组腺病毒载体的构建和鉴定
2.Construction of recombinant adenovirus vector encoding NF-κB RNA aptamer;核转录因子κB RNA适体重组腺病毒载体的构建
3.Construction of recombinant adenovirus vector pAdBM5-GFP-hIL-2 and the detection of viral titer重组腺病毒载体pAdBM5-GFP-hIL-2的构建及病毒滴度测定
3)recombinant adenoviral vector重组腺病毒载体
1.Construction and identification ofrecombinant adenoviral vector co-expressing connexon 43 and green fluorescent protein;Cx43和GFP双基因共表达重组腺病毒载体的构建和鉴定
2.Construction ofrecombinant adenoviral vector encoding human BMP-2 and detection of its biological activities;人骨形态发生蛋白2重组腺病毒载体的构建及其生物学活性检测
3.On the basis of AdEasy Adenoviral System,a simple and effective method for constructing therecombinant adenoviral vector encoding the antigen of M.以AdEasy腺病毒载体系统为基础,建立了一套简单有效构建编码结核分枝杆菌抗原的重组腺病毒载体的方法。
4)adenovirus vector重组腺病毒载体
1.ASB7-H1 and adenovirus genomic plasmid pBHGloxΔE1, 3Cre were cotransfected into 293 cells to construct antisense B7-H1adenovirus vector, named as Ad.结果穿梭质粒经酶切、凝胶电泳和测序鉴定与预期结果一致,重组腺病毒载体经PCR扩增后得到564bp和872bp特征性片段;其病毒滴度达:5×1010pfu/ml。
5)recombinant adenovirus重组腺病毒载体
1.Construction and expression ofrecombinant adenovirus containing human thymic stromal lymphopoietin gene;人胸腺基质淋巴生成素基因重组腺病毒载体的构建及表达
2.Construction of Recombinant Adenovirus Vector Ad. TCRα12-2-IRES-Vβ7.1 and Study Its Cytotoxicity to Hepatoma;共表达TCRα12-2和TCRVβ7.1重组腺病毒载体的构建及其杀伤肝癌细胞作用的研究
6)Recombinant lentiviral vector重组慢病毒载体
延伸阅读
猿猴病毒40载体分子式：CAS号：性质：也可简称为SV40载体。由于SV40病毒基因组具备：(1)由单一的共价闭合的环状DNA所组成；(2)含有多种功能的基因组的区域已正确定位；(3)病毒基因组能够自我复制。而可用作载体。野生型病毒DNA加入外源DNA后会导致DNA分子过大需缺失部分非必需DNA后亦用作载体。现在某些质粒型表达载体带有来自SV40的个别调控区，但不含病毒组的大部分编码区。通常采用的调控区是SV40DNA中的一个300bp的区段，位于病毒早期与晚期转录单位之间，含有一系列各不相同的顺式调控要素。这些调控要素包括：(1)DNA复制起始点；(2)早期与晚期mRNA转录的起始点和启动子；(3)能够激活复制起始点并可自动调节早期转录的T抗原结合位点；(4)可被细胞转录因子识别的一般由3个G/C丰富的21bp同向重复区组成的序列；(5)组成SV40早期增强子的两段72bp同向重复序列。除此之外，某些以SV40为基础的质粒载体因带有完整的SV40转录单位，激活源细胞SV40复制起始点，也可以编码SV40大T抗原。
